Generator
LacI:Cpn10
Part:BBa_K538200:Design
Designed by: Bas Stringer Group: iGEM11_Amsterdam (2011-09-08)
pLacI + RBS + Cpn10
This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538000.
For more information about the subparts' purpose and design, refer to their main page or design page, respectively.
- BBa_R0010
- BBa_B0034
- BBa_K538000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 278